Department of Biology
UNCG Confocal Microscopy Facility
New
Phone 336-334-4029
The UNCG confocal microscopy facility is located in NSB 353. The facility provides access to confocal microscopy equipment and image processing resources. Access is on a fee for use basis.
This confocal microscope was purchased and
supported by grant DBI-0319021 from the National Science Foundation, grant
2003-IDG-1011 from the
Facility Resources
Olympus
FLUOVIEW FV5OO/IX81
Olympus IX81 Motorized Inverted Microscope
Prior Scientific ProScan™ Motorized Stage including
Software integrated Stage Mapping
Available Objectives:
Position 1: UPlanApo 10x/0.40 infinity 8/0.17
Position 2: UPlanApo 20x/0.70 infinty 8/0.17
Position 3: UPlanFL 40x/1.30 Oil infinity 8/0.17
Position 4: PlanApo 60x/1.40 Oil infinity 8/0.17
Position 5: Open
Position 6: UPlanApo 40x/0.85 infinity 8 0.11-0.23
Available for use upon request:
UplanApo 60x/1.20 Water infinity 8 0.13-0.21
UIS Fluorescence Mirror
units:
DICT, TRITC, DAPI, CY5, FITC
Available lasers:
Multi-line Argon laser (457nm, 488nm, 514nm)
Green Helium Neon laser (543nm)
Red Helium Neon laser (633nm)
Blue Diode laser (405nm)
Supplemental Analysis Software:
Olympus Microsuite FIVE
A separate imaging workstation with Fluoview review software and Microsuite software is available for use.
Policies
Sign up policies for internal and
extramural users:
All policies are
subject to change without notice.
•All extramural users are required to make payment
arrangements, booking arrangements and submit a usage agreement prior to their
first session.
•All extramural user time must be negotiated prior to their first
booking.
•The confocal committee will negotiate all usage problems.
•Internal and extramural users may
sign up for available time slots a maximum of 48 hours and minimum of 2 hours
prior to use. Sign up for a time slot by contacting the facility manager with
your request. If you are unable to utilize your reserved time please inform the
facility manager immediately so you can be removed from the schedule.
•All students and Researchers must complete a training session(s)
specific to our facility to be approved to use the facility.
•All students and Researchers must be approved to use the facility prior
to requesting time.
•Facility hours of operation are Monday-Friday 8:00 AM – 5:00 PM.
The facility is closed during all of the recognized university holidays. After
hours access to the facility is available to approved users only.
•Primary investigators to include: UNCG Faculty and all Extramural
Researchers are fully responsible for their students and or technical staff
using the facility.
•Please report any mechanical or technical issues with facility equipment
to the facility manager immediately via voice mail. This applies to all
emergencies occurring at anytime. Dial 256-0574 (6-0574)
Some general confocal facility Do’s & Don’ts that should be practiced:
•Do not touch anything on the shelf
the multi line laser is sitting on
•Do cover the scope after use! Do not cover the epifluorescent unit until
it is completely cool!
•Do not change any instrument settings in the software that will not be
reset to the default
value after closing the program
•Do be careful with the use of immersion oil. The 40x & 60x located
in positions 3 & 4 on the
nosepiece are both oil immersion objectives. The 40x in position 6 is a high
dry.
•Do leave the multi line argon laser cooling fan on. This will cut itself
off after 3 minutes.
Then the toggle switch may be turned off.
•Please leave the facility as you found it. Clean and Straight
•If you are unsure about the proper use of any of the equipment in the
confocal microscopy facility please ask some one before you make a costly
assumption.
Facility resource fees
•All outside users must make
arrangements for scheduling, billing and payment prior to using the facility
•All fees are charged on a per minute basis.
•The fees listed below are charged to UNCG user accounts quarterly as of
10/26/04
Updated 6/1/2006
|
User |
Hourly Rate |
To include: |
|
UNCG Internal |
$30.00 |
Limited technical instruction and supervision |
|
UNCG Internal |
$60.00 |
Technical assistance |
|
Extramural user |
$110.00 |
Limited technical instruction and supervision |
|
Extramural user |
$170.00 |
Technical assistance |
Resource
booking
If you are an internal user interested in booking time please contact the
facility manager to do so.
NEW! Current Announcements Here you will find current information pertaining to reported equipment and software issues.
Current
users may log in to Calcium here. After log in you will be able to view both
calendars and book available time slots.
View
FLUOVIEW 500 availability
View
the imaging workstation availability
Images
The following gallery images have been generated by student and research faculty users of the UNCG Confocal Microscopy Facility. If you would like more information concerning our UNCG Biology department Research Faculty and research opportunities in their labs please visit our Research Faculty interest page. The image gallery will open in a new window.
Facility contacts
Faculty
John E. Tomkiel, Ph.D.
Paul A.
Steimle,Ph.D.
Dennis R. LaJeunesse , Ph.D.
Ron F. Morrison , Ph.D.
Technical Staff
/ Facility Manager / Emergency Contact
Anna G. Smith
Office Phone (336) 256-0574