| Norman H. L. Chiu |
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Title |
Assistant Professor |
| Expertise |
Bioanalytical Chemistry for Biomarkers |
| Education |
- B.Sc. in Chemistry, University of Liverpool, U.K., 1990
- M.Sc. in Analytical Chemistry, University of Bristol, U.K., 1992
- Ph.D. in Biochemistry, University of Windsor, Canada, 1993-1998
- Postdoctoral Fellow, Boston University, 1998-2001
- Postdoctoral Fellow and Scientist, Sequenom Inc, 1998-2001
- Assistant Professor, Northeastern University, 2001-2005
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| Office |
Sullivan Science Building, Rm 410 |
| Phone |
336.334.3126 |
| E-Mail |
Email Dr. Chiu |
| Group Website |
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One of the limiting factors for studying various biological systems is how well the systems can be monitored. The focus of Dr. Chiu’s research group is to identify specific analytical challenges on measuring different biomarkers, and develop new analytical methods for measuring those biomarkers. An interdisciplinary approach that includes analytical chemistry, molecular biology and/or nanotechnology is being used.
Dr. Chiu’s achievements included the development of (i) a novel expressible enzyme-coding DNA label for improving the sensitivity of immunoassay and its application on the detection human prostate specific antigen; (ii) a new commercial high throughput genotyping assay for rapid identification of bacteria using MALDI-TOF mass spectrometry; (iii) a non-specific enzymatic method for analyzing DNA sequences by using MALDI-TOF mass spectrometry; and (iv) an enzyme-based method for monitoring the performance of a bioreactor for wastewater treatment.
The objective of our current projects is to study the in vivo effects of various genotoxic chemicals that are commonly found in our environment and/or diets. Specific projects include the preparation of DNA standards with known modifications, the use of high throughput MALDI-TOF mass spectrometry (MS) to determine the identity and the amount of DNA modifications. Additionally, we are interested to apply the state-of-the-art imaging mass spectrometry (IMS) to monitor DNA modifications. In a collaboration with Prof. Bakhos Tannous at the Harvard Medical School, we are also using LC-MS to identify new disease biomarkers.
LIST OF PUBLICATIONS
- Christopoulos TK, Chiu NH. Expression Immunoassay. Antigen quantitation using antibodies labeled with enzyme-coding DNA fragment. Anal. Chem., 67; 4290 (1995).
- Chiu NH, Christopoulos TK. Hybridization assays using as a label an expressible DNA fragment encoding firefly luciferase. Anal. Chem., 68; 2304 (1996).
- Chiu NH, Christopoulos TK, Peltier J. Sandwich-type DNA hybridization assays based on enzyme amplified time-resolved fluorometry. Analyst, 123; 1315 (1998).
- White SR, Chiu NH, Christopoulos TK. Expression immunoassay based on antibodies labeled with a DNA fragment encoding the alpha-peptide of beta-galactosidase. Analyst, 123; 1309 (1998).
- Chiu NH*, Cantor CR. Mass spectrometry of nucleic acids. Clin. Chem., 45; 1578 (1999).
- Chiu NH, Christopoulos TK. Two-site expression immunoassay using firefly luciferase-coding DNA label. Clin. Chem., 45; 1954 (1999).
- Chiu NH*, Tang K, Yip P, Braun A, Koster H, Cantor CR. Mass spectrometry of single-stranded restriction fragments captured by an undigested complementary sequence. Nucleic Acids Res., 28; e31 (2000).
- White SR, Chiu NH, Christopoulos TK. Expression immunoassay. Methods, 22; 24 (2000).
- Leushner J, Chiu NH. Automated mass spectrometry: a revolutionary technology for clinical diagnostics [review]. Mol. Diagn. 5; 341(2000)
- Shahgholi M, Garcia BA, Chiu NH, Heaney PJ, Tang K. Sugar additives for MALDI matrices improve signal allowing the smallest nucleotide change (A:T) in a DNA sequence to be resolved. Nucleic Acids Res., 29; e91 (2001).
- Tannous B, Chiu NH, Christopoulos TK. Heterobifunctional linker between antibodies and reporter genes for immunoassay development. Analytica Chimica Acta, 459, 169 (2002).
- Wintzingerode FV, Bocker S, Schlotelburg C, Chiu NH, Storm N, Jurinke C, Van den Boom D. Base-specific fragmentation of amplified 16S rRNA genes and mass spectrometry analysis: A novel tool for rapid bacterial identification. Proc. Nat. Acad. Sci ., 99, 7039 (2002).
- S. Schiavo, A. El-Shafey, N. Jordan, C. Orazine, Y. Wang, X. Zhou, N.H. Chiu, and I.S. Krull. Immuno-PCR: an overview of the analytical technique currently pushing the limits of detection [review]. PharmaGenomics. January, 36-45 (2004).
- Li G, Zhou X, Wang Y, El-Shafey A, Chiu NH, and Krull IS. Capillary isoelectric focusing and affinity capillary electrophoresis approaches for the determination of binding constants for antibodies to the prion protein. J. Chromatogr A, 1053(1/2), 253-262 (2004).
- Schiavo S, Yang W, Chiu NH and Krull IS. Comparison of fluorometric detection methods for quantitative PCR. J Immunoassay Immunochem, 26(1), 1-12 (2005).
- Littman M, Chiu NH and Wang J. Specific recognition of non-denatured nitrite-oxidizing system of Nitrospira moscoviensis by monoclonal antibody Hyb 153-3. J Chem Technol Biotechnol 81 (3), 318-21 (2006).
- Qing L, Chiu NH and Vouros P. Investigation of benzonase/alkaline phosphatase digestion of DNA and DNA-adducts using LC-ESI MS. Anal. Chem., 79(5), 1907-17 (2007).
- Barnes CA, and Chiu NH. Accurate characterization of carcinogenic DNA adducts using MALDI tandem time-of-flight mass spectrometry. Int. J. Mass Spectrom. In Press.
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