 310
Stone Building
P.O. Box 26170
University of North Carolina at Greensboro
Greensboro, NC 27402-6170
Phone (336) 334-5313 • Fax (336) 334-4129
e-mail: jim_fleet@uncg.edu
Ph.D., Cornell University, 1988
R E S E A R C H I N T E R E S T S
Mineral metabolism; mechanisms of intestinal calcium and irontransport;
biology of aging; mechanisms of bone formation; genetic influences on mineral
metabolism; the biology of vitamin D actions on intestinal cells.
C U R R E N T R E S E A R C H
I am currently conducting studies that examine the role of specific proteins
in the regulation of intestinal calcium and iron absorption using a cell
culture system, the human colonic carcinoma cell line, Caco-2. My current
approach is to insert fusion genes into the Caco-2 cells that can over- and
under-express proteins that are proposed to be essential for the specific,
regulated transport of calcium or iron across the absorptive enterocyte.
The proteins include: (for calcium) calbindin D9k, the vitamin D receptor,
the retinoid X receptor subtypes, and calreticulin ,(for iron) ferritin H
and L chains, the hemochromatosis gene (HLA-H), and the gene for the iron
transporter (DCT1). I am also studying the role of the vitamin D receptor
and the hemochromatosis gene using gene knockout mice.
R E C E N T P U B L I C A T I O N S
Fleet, J.C. and Wood, R.J. (1994) Regulation of calbindin D-9k mRNA levels
by 1,25 dihydroxyvitamin D3 in the human colonic carcinoma cell line, Caco-2.
Arch. Biochem. Biophys. 308: 171-174.
Fleet, J.C., Bruns, M.E., Hock, J.M. and Wood, R.J. (1994) Growth hormone
and parathyroid hormone stimulate intestinal calcium absorption in aged female
rats. Endocrinology 134: 1755-1760.
Fleet, J.C. and Hock, J.M. (1994) Identification of osteocalcin mRNA in
non-osteoid tissue of rats and humans by RT-PCR. J. Bone Min. Res. 9:
1565-1573.
Kim, Y., Christman, J.K., Fleet, M.C., Cravo, M.L., Salomon, R.N., Smith,
D., Ordovas, J., and Mason, J.B. (1995) Moderate folate deficiency does not
cause either global or c-myc specific hypomethylation of hepatic and colonic
DNA in rats. Am. J. Clin. Nutr. 61: 1083-1090.
Fleet, J.C., Wood, R.J., Harris, S., Dawson-Hughes, B. (1995) The Bsm-1 vitamin
D receptor restriction fragment length polymorphism (BB) predicts low bone
density in premenopausal black and white women. J. Bone Min. Res.
10: 985-900.
Fleet, J.C., Bradley, J., Reddy, G.S., and Wood, R.J. (1996) 1a,25(OH)2 Vitamin
D3 analogs with minimal in vivo calcemic activity can stimulate significant
transepithelial calcium transport and mRNA expression in vitro. Arch.
Biochem. Biophys. 329: 228-234.
Fleet, J.C., Cashman, K., Cox, K., and Rosen, V. (1996) The effects of aging
on the bone inductive activity of recombinant human bone morphogenetic protein-2.
Endocrinology 137: 4605-4610.
Wood, R.J., Fleet, J.C., Cashman, K., Wilkening, C. and DeLuca, H.F. (1998)
Age-associated intestinal resistance to the action of 1,25
(OH)2 vitamin
D3 in rats. Submitted to
Endocrinology.
Han, O., Fleet, J.C., Wood, R.J. (1998) Reciprocal
regulation of HFE and Nramp2 gene expression by iron in human intestinal
cells. Submitted to Journal of Biological
Chemistry.
Fleet, J.C., and Wood, R.J. (1998) Further
characterization of Caco-2 cells as a model for vitamin D mediated
intestinal calcium transport. Submitted to Journal of
Nutrition.
Wood, R.J. and Fleet, J.C. (1997) The genetics of
osteoporosis: Vitamin D receptor polymorphisms. Annu. Rev. Nutr. (in
press).
Copyright©1997
The University of North Carolina
at Greensboro
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