BIOLOGY 280

FUNDAMENTALS OF MICROBIOLOGY

4.0 Credit Hours Fall 2010

Pre-requisites: BIO 111 (with a C- or better) and passing grade in either BIO 271 or BIO 277

Co-requisite: BIO 280 Lab must be taken concurrently with BIO 280 Lecture

For Whom Planned: Welcome! This course is intended to serve as a survey course for those nursing, nutrition, and biology majors who will not need any further courses in the field. It is also recommended for those planning to pursue further education in P.A., pharmacy, or dental schools, or nutrition. This microbiology course is designed to give you a working understanding of microbes and their interactions with humans…and most importantly, how they impact your own everyday life. This information will be useful for medical careers and food service careers, as well as making you a more informed and safer consumer throughout your life.

Instructor: Robin Maxwell Office Hours (Rm.325 SSB): Tu 11:00, Th 2:00, Fri 9:00 or by appointment Office Phone: 256-0066 E-mail: Robin_Maxwell@uncg.edu

Required Texts: Microbiology with diseases by taxonomy; 3^rd edition, Bauman

Microbiology Lab Exercises; 4^th edition, Maxwell

Catalogue Description: General survey of microscopic life and its impact on medicine, public health, and the environment. Includes laboratory work with bacteria, emphasizing aseptic technique. Students cannot receive credit for both this course and BIO 481.

Student Learning Outcomes: On completion of this course, the student will be able to characterize the major bacterial and viral species by physical characteristics, biochemical characteristics, growth characteristics, virulence factors and diseases caused. Students will understand the principles of basic immunology and differentiate between the responses of the cell-mediated and humoral systems of the body to pathogen invasion. Students will be able to apply the principles of epidemiology to novel situations and diseases to both predict and propose solutions for epidemic, pandemic, or endemic infectious diseases of man.

Attendance Policy: ATTENDANCE IN LAB IS MANDATORY!! Three absences (excused or unexcused) will result in your being dropped from the course. Arriving in lab more than 15 minutes late is recorded as an absence, as is leaving lab before all work is completed. Closed-toe shoes are required for lab attendance, with points removed from the lab average for each infraction. THERE ARE NO MAKEUP LABS!!! Attendance in lecture is encouraged, because lecture quizzes closely adhere to material covered in class, and participation grades may be earned only when you are in class.

Evaluation Methods: The lecture portion of the course will count as two-thirds of course grade, and will be calculated on the basis of four lecture quizzes and the final exam (90%) and class participation grade (10%). (Note: The final is a cumulative exam, which will count the equivalent of TWO quizzes.) The lab portion will count as one-third of the overall grade in the course. The lab grade will be based on three lab tests (80%) and three unknown culture identifications (20%). Lab tests will include a written and a practical portion. Unknown submissions will be on the form given in the lab manual, and will be graded on a basis of 10, with 10 being the highest possible score. The Academic Integrity Policy must be signed on all graded assignments. Makeup lecture or lab tests, given with valid excuse only, will be essay format.

Gradebooks and all resources for the course are available on Blackboard.

GRADING: I use a 10 point scale, with plus or minus grades given only in the top 2 points of each letter grade:

*Plus and minus grades are possible only for those within 2 points of the letter grade border, and will be determined by the class participation grade. (If you have earned 100% or more of the class participation points, you get the next letter grade up with a minus. With less than 100%, you stay at the letter grade earned, with a plus added.)

*98-100 = A or A+

90-98 = A

*88-89 = A- or B+

80-87= B

*78-79 = B- or C+

70-77 = C

*68-69 = C- or D+

60-67 = D

below 60 = F

Course Schedule: This is a tentative syllabus. We may adapt the topics and readings to fit the time and interests of the class.

DATE LECTURE TOPIC *READING LAB EXER. LAB TOPIC

Aug.	23M	Introduction - What is Microbiology?	Ch. 1	1, 2	Ubiquity, Hand washing
	25W	History of Microbiology	Ch. 1, p. p.411-413	3	Microscopy
	30 M	Bacterial Anatomy	Chapter 3, p. 183-185, 313-319	4	Bacterial Staining
Sept.	1W	Bacterial Anatomy and Growth		5	Gram Stain
	6M	LABOR DAY HOLIDAY	-----------------	------------	-------------------------------
	8W	Viral Anatomy	Chapter 13	8, 9	Negative, Capsule Stains
	13M	Viral Replication		6, 7	Acid-Fast, Spore Stains
	15W	--LECTURE QUIZ 1--	--------------	10	Unknown #1 workday
	20M	Viruses and Cancer	Chapter 13, p. 22	--LAB TEST 1, Unk. due--
	22W	Prion diseases		11, 12	Transfer and Isolation
	27M	Vertical and Horizontal Gene Transfer – Transformation	Chapter 7	13	Cultural Characteristics
	29W	Horizontal Gene Transfer – Conjugation and Transduction		14, 15	Temperature, Oxygen Needs
Oct.	4M	Host - Microbe Interactions, The Establishment of Disease	Chapter 14	34	Transformation Exp.
	6W	Disease types and Epidemiology		17, 18	Antiseptics, Disinfectants, Antibiotics, and Radiation
	11M	FALL BREAK	---------------	------------	--------------------------------
	13W	--LECTURE QUIZ 2--	------------------	16	Osmotic Effects, Record and discuss old results
	18M	Weapons of the Invaders	Chapter 14, cont.	36	Transmission of Infection
	20W	Host Defenses, First and Second Line	Chapter 15	--LAB TEST 2 --
	25M	Host Defenses, Second & Third Lines		20, 21	Exoenzymes, Fermentations
	27W	Third line: Cell-Mediated Immunity	Chapter 16	22, 30	Enriched, Differential & Selective Media, Get Unknowns # 2 & 3
Nov.	1M	Third line: Humoral Immunity		23	ID. of Staphylococci
	3W	Immunology: Allergy, Vaccine product.	Chapter 17 (part), Chapter 18 (part)	24	ID. of Streptococci
	8M	--LECTURE QUIZ 3--	-----------	25, 26	Oxidase, H2S Production, Motility
	10W	History and Scope of AIDS	p. 719-726, web reading	27 28	TSIA Medium IMViC tests
	15M	HIV disease and treatment		30, Chart	Unknown and record work
	17W	Influenza	p. 733-737, web reading	35	Serology, Unk. due
	22M	Bioterrorism: Smallpox, Anthrax	p. 545-547, 682-685, web reading	---LAB TEST 3--
	24W	THANKSGIVING BREAK	---------------	------------	--------------------
	29M	Herpes viruses - simplex, zoster, etc.	p. 684-694	31	Water Analysis
Dec.	1W	Hepatitis viruses - A, B, C, D, E	p. 698-701, 711-712, 716-717, 738	32	Food Analysis
	6M	Quiz 4 (includes labs 31, 32, 33)	---------------	33	Food Production, Checkout
	13M	LECTURE FINAL -- 3:30-6:30pm	-----------------	------------	-----------------

*Note: Although these specific assignments are needed for particular lectures, the entire text is used in this class!

Additional Readings to be done as needed: (not specifically listed above)

· Chapter 2 (Chemistry of Microbiology) - for those who have had no chemistry at the college level, or feel rusty.

· Chapter 4 (Microscopy, Staining, 7 Classification) - to accompany Labs 3 - 10.

· Chapter 5 (Microbial Metabolism) - to accompany Labs 20 - 30.

· Chapter 6 (Microbial Nutrition and Growth) - to accompany Labs 11 - 15, & 22.

· Chapter 9 (Controlling Microbial Growth in the Environment) - to accompany Labs 16 - 18.

· Chapter 10 (Controlling Microbial Growth in the Body: Antimicrobial Drugs) - to accompany Lab 17.

· Chapter 17 (Immunization and Immune Testing) - to accompany Lab 35

· Chapters 19-25 (Chapters on specific types of microbial pathogens) - to accompany Labs 22 - 28.

· Chapter 26 (Applied and Environmental Microbiology) - to accompany Labs 31 - 33.

Microbiology Ground Rules

I assume you are all responsible adults and will act accordingly in class and lab. However, remember the old saying about making assumptions?! Just so we are all on the same page, with no nasty surprises, the following are the minimum standards for attending Microbiology lecture and lab. If you wish to remain in the course, initial the top of the "Student Information" sheet to acknowledge that you are aware of and agree to abide by the expectations in this class.

Lecture:

1. Students should be respectful of the classroom environment. The following behaviors will result in a student being asked to leave class, either for the day, or for good:

· Texting on cell phones during class

· Using a laptop in class for anything other than taking notes

· Talking during class (except during group assignments, or to ask questions)

2. Arrive on time, and stay for the entire class period. A brief break will be given about halfway through the class, for students to make phone calls, smoke, get snacks, or use the rest room.

3. If you do not attend class, you are responsible for finding out what you missed, and trying to get notes from another student. I will not re-teach the material to you alone.

4. Students should check over their tests carefully with the posted keys, and point out any potential grading errors to the instructor within one week of receiving the test back. After this point, grades will not be altered.

5. In-class group activity grades are earned only by students who are actually in class on that day. Check on Blackboard to make sure you have points recorded for you when papers are returned, and notify me within one week of the activity if you do not see your points.

6. Final course grades are assigned EXACTLY according the scale posted. Fairness dictates that you will receive the grade you earn, and will not be given any extra points for "working hard". Everyone will work hard in this class, but performance will determine your grade.

Lab:

1. Lab attendance is mandatory. Three absences (excused or unexcused) will result in your being dropped from the course, and if this happens after the drop date, a WF will be assigned. Arriving at lab more than 15 minutes from the start time will be recorded as an absence. Leaving lab before all of the assigned work is completed will be recorded as an absence.

2. Make-up labs are not possible, due to the nature of the live materials and perishable supplies. While we may be able to save some cultures for you to look at later, there is no time or personnel available to teach the entire lab to a single person.

3. Closed-toe shoes must be worn at all times in the lab room. Shoes that show any portion of the toes are unacceptable (this includes, but is not limited to: sandals, peep-toe shoes, crocs, shoes that show toe cleavage). One grace violation will be allowed, but after this, each infraction will result in a reduction of the student's final lab average by one point.

4. If you know in advance that you cannot attend your normal lab, you may be able to attend the other lab, at the discretion of the instructor, which will not count as an absence. Please ask your lab instructor about this possibility as soon as you realize the need to switch.

5. Much of the lab work done will be group work. Each student is expected to pull their weight, not pass off work to their partners. Not only your grade, but the entire group's safety depends on your work ethic and cooperation.

STUDENT INFORMATION

(print out and turn in on the first day of classes)

Full name: (Also what you want to be called, if that is not what your mother named you)

Contact phone number and email address:

Major and Career Aspirations:

Science courses taken at the college level: (if taken somewhere other than UNCG, please indicate where taken)

Anything else I need to know about you: (learning disabilities, medical conditions that may impact on this course, travel issues, child care issues, etc.)